D6293

pUC18 DNA Hae III Digest

for DNA electrophoresis

• UNSPSC-Code: 12352202
• NACRES: NA.25

Eigenschaften

• Form: liquid
• Verwendung: 200 uses (0.1 mg size); 40 uses (20 μg size)
• Eignung: suitable for electrophoresis (DNA)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Anwendung

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.[1]

Komponenten

Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Sonstige Hinweise

Contains 11 fragments, 11–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Angaben zur Herstellung

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable