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D4668

Sigma-Aldrich

Dextranase from Penicillium sp.

lyophilized powder, 100-250 units/mg protein

Synonym(e):

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

CAS-Nummer:
9025-70-1
EC-Nummer:
3.2.1.11 (BRENDA, IUBMB)
EG-Nummer:
232-803-9
MDL-Nummer:
MFCD00130940
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

200

Spezifische Aktivität

100-250 units/mg protein

Zusammensetzung

Protein, ~25% Lowry

Lagertemp.

2-8°C

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Anwendung

Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure.

Biochem./physiol. Wirkung

The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Einheitendefinition

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Physikalische Form

Partially purified, lyophilized powder

Sonstige Hinweise

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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T Pons et al.
Proteins, 31(4), 345-354 (1998-06-17)
The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. Sequence analysis has revealed only one homologous sequence, CB-8 protein, from Arthrobacter sp., with 30% sequence identity.
Timothy D Leathers et al.
Biotechnology letters, 31(2), 289-293 (2008-10-22)
Alternan is a unique glucan with a backbone structure of alternating alpha-(1 --> 6) and alpha-(1 --> 3) linkages. Previously, we isolated strains of Penicillium sp. that modify native, high molecular weight alternan in a novel bioconversion process to a
Dextranase in sugar industry: A review
Efrain Rodriguez Jimenez
Sugar Tech, 11(2), 124-134 (2009)
Keiji Tsusaki et al.
Carbohydrate research, 344(16), 2151-2156 (2009-09-11)
The bacterial strain PP710, isolated from soil and identified as Paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. This alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of
Ndegwa Henry Maina et al.
Biomacromolecules, 12(2), 409-418 (2011-01-07)
Weissella confusa VTT E-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked D-glucosyl units and very few α-(1→3) branch linkages. A mixture of the Chaetomium erraticum endodextranase and the Aspergillus niger α-glucosidase was used to
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