Fortfahren mit
C1403
Cholesterin-Esterase aus Pseudomonas sp.
lyophilized powder, ≥200,000 units/g protein
Synonym(e):
Cholesterin-Esterase aus Pseudomonas fluorescens, Sterolester-acylhydrolase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 units | Warenkorb auf Verfügbarkeit prüfen | CHF 247.00 |
100 units | Warenkorb auf Verfügbarkeit prüfen | CHF 794.00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-808-6
MDL number:
Specific activity:
≥200,000 units/g protein
Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Segment
specific activity
≥200,000 units/g protein
mol wt
~300 kDa
composition
protein, ≥40% biuret
storage temp.
−20°C
General description
Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
Application
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Biochem/physiol Actions
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.[1]
Other Notes
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.
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Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥200,000 units/g protein | specific activity ≥10,000 units/g protein | specific activity ≥200 units/g protein | specific activity ≥100 units/mg protein, ≥70 units/mg dry wt. |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~300 kDa | mol wt ~129 kDa | mol wt 84 kDa by SDS-PAGE | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| composition protein, ≥40% biuret | composition Protein, ~20% | composition protein, ≥20% | composition - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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