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C0528

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type III, essentially salt-free, lyophilized powder

Synonym(e):

FITC-casein

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10 MG
CHF 167.00
25 MG
CHF 337.00
100 MG
CHF 1’000.00

CHF 167.00


Voraussichtliches Versanddatum28. Mai 2025


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10 MG
CHF 167.00
25 MG
CHF 337.00
100 MG
CHF 1’000.00

About This Item

MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.32

CHF 167.00


Voraussichtliches Versanddatum28. Mai 2025


Bulk-Bestellung anfordern

Typ

Type III

Form

essentially salt-free, lyophilized powder

Kennzeichnungsgrad

≥50 μg FITC per mg solid

Löslichkeit

water: 5 mg/mL, clear to hazy

Lagertemp.

2-8°C

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Anwendung

Casein fluorescein isothiocyanate from bovine milk has been used:
  • in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment[1]
  • to make a reference line in immunochromatographic devices[2]
  • in running gel for the documentation of proteinases[3]

highly sensitive protease substrate

Biochem./physiol. Wirkung

Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.[4]

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Tomasz Chamera et al.
Journal of molecular biology, 431(11), 2180-2196 (2019-04-27)
Hsp104 is a yeast chaperone that rescues misfolded proteins from aggregates associated with proteotoxic stress and aging. Hsp104 consists of N-terminal domain, regulatory M-domain and two ATPase domains, assembled into a spiral-shaped hexamer. Protein disaggregation involves polypeptide extraction from an
Yong Tang et al.
PLoS biology, 17(2), e3000136-e3000136 (2019-02-07)
Endosymbiosis has driven major molecular and cellular innovations. Plasmodium spp. parasites that cause malaria contain an essential, non-photosynthetic plastid-the apicoplast-which originated from a secondary (eukaryote-eukaryote) endosymbiosis. To discover organellar pathways with evolutionary and biomedical significance, we performed a mutagenesis screen
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food
Freese D, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 162(4), 66-72 (2012)

Artikel

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Questions

  1. What's the mwt of fitc-casein in kda?

    1 answer
    1. The Casein used to prepare this product is a mixture of Alpha-s1, Alpha-s2, Beta, and Kappa Casein. Combined, these forms of casein have a molecular weight range of 19 - 25.2 kDa. The Fluorescein isothiocyanate I isomer has a molecular weight of 389.38. The ratio of casein to FITC is approximately 11:1. A confirmed molecular weight has not been established.

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