A4268

α-Amylase aus Schweinepankreas

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

• Synonym(e): β-N-Acetylglucosaminidase, Schweineplazenta, PPA, Schweinepankreas α-Amylase, al1,4-Glucan-4-glucanohydrolase,
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• MDL number: MFCD00081319
• EG-Nummer: 3.2.1.1 (BRENDA, IUBMB)
• Specific activity: 700-1400 units/mg protein (E1%/280)
• Biological source: Porcine pancreas

Eigenschaften

• biological source: Porcine pancreas
• Quality Segment: 300
• type: Type I-A
• form: saline suspension
• specific activity: 700-1400 units/mg protein (E1%/280)
• mol wt: 51-54 kDa
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): activity assay: suitable
• suitability: suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
• application(s): diagnostic assay manufacturing
• greener alternative category: , Enabling
• storage temp.: 2-8°C

Beschreibung

General description

Molekülmasse: 51–54 kDa.
α-Amylase, isoliert aus Schweine-Pankreas, ist ein Glykoprotein. Sie ist eine einzelne Polypeptidkette von ~475 Resten, die zwei SH-Gruppen und vier Disulfidbrücken sowie dicht gebundenes Ca²⁺ für die Stabilität enthält. Chloridionen sind notwendig für Aktivität und Stabilität. Der pH-Wertbereich für Aktivität beträgt 5,5 bis 8,0, wobei der optimale pH-Wert bei 7 liegt.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [1].

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability.

Physical form

Suspension in 2.9 M NaCl-Lösung, enthält 3 mM CaCl_2.

Preparation Note

2x kristallisiert

Other Notes

1 U setzt 1.0 mg Maltose aus Stärke in 3 Minuten frei bei pH 6.9 und 20 °C.

Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves