A3449

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 19:1

• Synonym(e): 30% Acrylamide/Bisacrylamide 19:1, Polyacrylamide solution
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770824
• NACRES: NA.25
• MDL number: MFCD00080848

Eigenschaften

• Produktname: Acrylamid/Bis-acrylamid, 30 % -Lösung, BioReagent, suitable for electrophoresis, 19:1
• sterility: 0.2 μm filtered
• Quality Segment: 200
• product line: BioReagent
• form: liquid
• feed ratio: 19:1
• technique(s): electrophoresis: suitable
• suitability: suitable for electrophoresis
• foreign activity: Protease, none detected
• storage temp.: 2-8°C
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Beschreibung

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Diese 30%-igen (w/w) Acrylamid-/Bis-Acrylamid-Lösungen sind in den gängigsten Verhältnissen erhältlich, die bei der Elektrophorese von Proteinen und Nukleinsäuren eingesetzt werden. Die Konzentration basiert auf dem Gesamtgewicht von Acrylamid und Bis-Acrylamid.

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Preparation Note

Aus Elektrophoresequalität Acrylamid und Bisacrylamid in "ultra-pure"-Qualität Wasser hergestellte Lösung.

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302 + H332,H315,H317,H319,H340,H350,H361f,H372
• pcodes: P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• target_organs: Peripheral nervous system
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter