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Sigma-Aldrich

Toluidinblau

8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)

Synonym(e):

Tolonium chloride; Basic Blue 17

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About This Item

Empirische Formel (Hill-System):
C15H16ClN3S · 0.5ZnCl2
CAS-Nummer:
6586-04-5
Molekulargewicht:
373.97
Farbindexnummer:
52040
Beilstein:
4068052
MDL-Nummer:
MFCD03548293
UNSPSC-Code:
12171500
PubChem Substanz-ID:
329769757
NACRES:
MA.02

Qualität

for microscopy (Hist., Vit.)

Qualitätsniveau

200

Assay

8.74% (ZN (THEORY))

Form

powder or crystals

Farbe

green to very dark green

Löslichkeit

methanol: 0.01 g/10 mL, blue to very deep blue

εmax

≥800 at 621-636 nm in water

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C

InChI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

InChIKey

DAQXTSXITPUGRG-UHFFFAOYSA-L

Allgemeine Beschreibung

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Anwendung

Toluidine Blue is suitable for use in the following applications:
  1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
  2. in metachromatic staining of sulfated mucins and related materials
  3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
  4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Biochem./physiol. Wirkung

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number
BCCK0876
BCCL0360
BCCH1582
BCCD9721
BCBZ9485

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