DGTP-RO

2′-Desoxyguanosin 5′-triphosphat Dinatriumsalz

PCR Grade, sodium salt

• Synonym(e): dGTP-Na₃
• Empirische Formel (Hill-System): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS-Nummer: 93919-41-6
• Molekulargewicht: 573.13
• UNSPSC Code: 12352302
• PubChem Substance ID: 329798919
• MDL number: MFCD00080337
• NACRES: NA.54
• Assay: 99% (dNTP)
• Form: solution
• Storage temp.: −20°C

Eigenschaften

• assay: 99% (dNTP)
• form: solution
• usage: sufficient for 125,000 standard reactions (03732703001), sufficient for 31,250 standard reactions (11969030001), sufficient for 6,250 standard reactions (11934538001)
• packaging: pkg of 1,250 μL (11969030001 [100 mM]), pkg of 4 × 1,240 μL (03732703001 [4x100 mM]), pkg of 250 μL (11934538001 [100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP), 100 mM
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChI key: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Beschreibung

General description

2′-Desoxyguanosin-5′-triphosphat (dGTP) besteht aus einer Guaninnukleobase, die an Desoxyribose gebunden ist, und einem 5‘-Hydroxyl an dem Zucker, der an eine Kette aus drei Phosphatsresten gebunden ist. Es wird von Zellen für die Synthese von DNA mithilfe von DNA-Polymerase verwendet.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Desoxyguanosin 5′-triphosphat-Trinatriumsalz wird bei Anwendungen wie der DNA-Sequenzierung, PCR und anderen DNA-Polymerase-basierten DNA-Synthesen und Reparaturmethoden eingesetzt.

Physical form

solution of dGTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Other Notes

For general laboratory use.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash