14-865

p53-GST (recombinant protein)

• Synonym(e): transformation related protein 53
• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Form: liquid
• Assay: ~95% (SDS-PAGE)
• Biological source: human
• Mol wt: M_w 72 kDa

Eigenschaften

• biological source: human
• Quality Segment: 100
• assay: ~95% (SDS-PAGE)
• form: liquid
• shelf life: 1 yr
• mol wt: M_w 72 kDa
• manufacturer/tradename: Upstate^®
• concentration: 0.5 mg/mL
• technique(s): activity assay: suitable
• solubility: water: soluble
• NCBI accession no.: NP_000537
• UniProt accession no.: P04637
• shipped in: dry ice
• Gene Information: human ... TP53(120407068)

Beschreibung

General description

Research area: Apoptosis

N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography. Product Source: Full-length human p53 protein, expressed in e. coli. p53 protein is encoded by the tumor suppressor gene TP53 gene mapped on chromosome 17.

Biochem/physiol Actions

In the cell, the p53 protein binds to DNA, leading to the activation of another gene that produces the p21 protein. This protein interacts with a cell division-stimulating protein (cdk2), preventing the cell from progressing to the next stage of cell division. Mutation in p53 protein leads to uncontrolled cell division resulting in tumor formation. The p53 protein is suggested to impede the phenotypic and genomic changes linked to cancer development through its intricate interaction with various signaling pathways including cell division, genomic stability maintenance, apoptosis, autophagy, immune response, and tumor microenvironment regulation.

Physical form

Glutathione agarose chromatography

Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.

Preparation Note

1 year at -20°C

Analysis Note

Routinely evaluated by SDS-PAGE and Coomassie Stain

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids