HPLC Analysis of Xylo-oligosaccharide on Proteomix^® SAX-NP5

column

Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)

mobile phase

(A) 0.05% ammonium hydroxide in acetonitrile (B) 0.05% ammonium hydroxide in water

gradient

15-60% B (20 min)

flow rate

0.7 mL/min

pressure

1015 psi (70 bar)

temp.

35 °C

detector

ELSD

injection

10 μL

sample

Xylose oligosaccharides