HPLC Analysis Carbohydrates on Proteomix^® SAX-NP5

column

Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)

mobile phase

[A] 0.05% (25% ammonium hydroxide) in acetonitrile; [B] 0.05% (25% ammonium hydroxide) in water

gradient

15 to 80% B in 20 min

flow rate

0.7 mL/min

temp.

35 °C

detector

ELSD

sample

Corn starch hydrolysate; glucose oligomer (G1 to G6)