The protocol will be on the lot-specific Certificate of Analysis.
Please see the example below:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0001_0204I0225.pdf
RAB0001
Human IgG ELISA Kit
for serum, plasma
Synonyme(s) :
IgG, Immunoglobulin-G
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
41116158
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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Laissez-nous vous aiderspecies reactivity
human
technique(s)
ELISA: suitable, capture ELISA: suitable
shipped in
wet ice
storage temp.
−20°C
General description
Immunoglobulin G (IgG) belongs to immunoglobulin family, and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weights of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds linking the two heavy chains, linking the heavy and light chains, and are present inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively. IgG is produced and released from CD5+ B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates. This assay employs a specific capture antibody coated on a 96 well plate.
Immunogen
Recombinant Human IGG
Application
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Cet article | |||
|---|---|---|---|
| description for serum, plasma | description affinity isolated antibody, buffered aqueous solution | description recombinant, expressed in proprietary host, clone SP137, affinity isolated antibody | description affinity isolated antibody, buffered aqueous solution |
| species reactivity human | species reactivity rat | species reactivity - | species reactivity monkey |
| technique(s) capture ELISA: suitable | technique(s) western blot: suitable, immunohistochemistry: suitable, indirect ELISA: suitable | technique(s) immunohistochemistry: 1:100 | technique(s) indirect ELISA: suitable, western blot: suitable, immunohistochemistry: suitable |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
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Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Classe de stockage
12 - Non Combustible Liquids
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Is there any specific description of the kit RAB0001? We would like to have s protocol do that we can test the IgG level in the patient sera.
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