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Key Documents

Q1126

Sigma-Aldrich

Q Sepharose Fast Flow

preswollen, 45-165 μm (wet), exclusion limit ~4,000,000 Da

Synonyme(s) :

Q Sepharose

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About This Item

Numéro MDL:
Code UNSPSC :
47101511
Nomenclature NACRES :
NA.56

Forme

preswollen

Niveau de qualité

Technique(s)

RNA extraction: suitable

Taille des particules

45-165 μm (wet)

Dimension de pores

~4,000,000 Da exclusion limit

Capacité

0.18-0.24 mmol Cl-/mL, packed gel

Température de stockage

2-8°C

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Description générale

−Ο−CH2CHOHCH2OCH2CHOHCH2N+(CH3)3

Application

Q Sepharose is used in protein chromatography, ion exchange chromatography and anion exchange media. Q Sepharose has been used to study compounds of plant defense with applications for natural control of phytopathogenic fungi. Q Sepharosehas also been used to develop an efficient method for extracting high-quality mRNA from soil and to study the immunomodulatory proteins from garlic (Allium sativum).

Forme physique

Preswollen in 20% ethanol

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

100.4 - 109.4 °F - closed cup

Point d'éclair (°C)

38 - 43 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Irini Bournazou et al.
The Journal of clinical investigation, 119(1), 20-32 (2008-11-27)
Apoptosis is a noninflammatory, programmed form of cell death. One mechanism underlying the non-phlogistic nature of the apoptosis program is the swift phagocytosis of the dying cells. How apoptotic cells attract mononuclear phagocytes and not granulocytes, the professional phagocytes that
E G Campos et al.
International journal for parasitology, 29(5), 655-662 (1999-07-15)
Cytochrome c peroxidase oxidises hydrogen peroxide using cytochrome c as the electron donor. This enzyme is found in yeast and bacteria and has been also described in the trematodes Fasciola hepatica and Schistosoma mansoni. Using partially purified cytochrome c peroxidase
Shikimate dehydrogenase from pepper (<I>Capsicum annuum</I>) seedlings. Purification and properties.
Diaz, J., and Merino, F.
Physiologia Plantarum, 100(1), 147-152 (1997)
Fatima Clement et al.
International immunopharmacology, 10(3), 316-324 (2009-12-17)
Garlic (Allium sativum), an important medicinal spice, displays a plethora of biological effects including immunomodulation. Although some immunomodulatory proteins from garlic have been described, their identities are still unknown. The present study was envisaged to isolate immunomodulatory proteins from raw
Carsten Mettel et al.
Applied and environmental microbiology, 76(17), 5995-6000 (2010-07-14)
Here, we report an efficient method for extracting high-quality mRNA from soil. Key steps in the isolation of total RNA were low-pH extraction (pH 5.0) and Q-Sepharose chromatography. The removal efficiency of humic acids was 94 to 98% for all

Chromatograms

application for HPLC

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