The supplier has not tested MAK459 with tissue samples, but it is possible that it would work. It's important to note that samples containing bile acids such as sodium deoxycholate can interfere with this assay and should be avoided. Additionally, samples should be clear and free of particles or turbidities.
MAK459
Detergent Assay Kit
Sufficient for 100 colorimetric tests
Synonyme(s) :
Detergent Detection Kit
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A propos de cet article
NACRES:
NA.84
UNSPSC Code:
12161503
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
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Laissez-nous vous aiderQuality Segment
storage condition
(No metal containers. Tightly closed.)
input
protein
water
soil
application(s)
environmental
detection method
colorimetric
storage temp.
2-8°C
General description
Detergents play a significant role in increasing environmental pollution across various environments. This pollution originates from wastewater sources, such as residential areas where household detergents are used, agricultural runoff containing herbicides and insecticides, and certain industries.In biochemical research, detergents are commonly used for membrane protein separation. However, they can interfere with protein assays by affecting protein-protein interactions, enzymatic activities, or protein structure. Detecting and quantifying the presence of detergents is essential for accurate measurements in protein assays. Detergents used during protein extraction or purification processes may carry over into downstream applications, such as electrophoresis, chromatography, or mass spectrometry. This is crucial for maintaining assay accuracy, assessing protein functionality, ensuring compatibility with downstream applications, and preserving sample integrity.
Application
The Detergent Assay Kit may be used for:
- Soil Testing
- Water Testing
- Detergent Detection in Biological Samples
The kit is suitable for detecting traces of detergent in purified protein samples and the determination of detergent in water and soil samples.
Biochem/physiol Actions
Simple, direct and automation-ready procedures for measuring detergent concentration in biological samples are very desirable. The Detergent Assay Kit is designed to measure detergent directly without any pretreatment. Above the critical micellar concentration, detergent forms micelle in solution that trap the colorimetric dye. The intensity of the color is directly proportional to the detergent concentration in the sample.
Features and Benefits
Efficient Procedure: Streamline your workflow with a hassle-free 15-minute process, requiring only the addition of a single working reagent and a small 20 μL sample size, saving you valuable time and effort.
Linear Detection Range:
Linear Detection Range:
- Tween 80: Detect concentrations ranging from 0.012 to 4 mM
- Tween 20: Detect concentrations ranging from 0.06 to 5 mM
- Triton X-100: Detect concentrations ranging from 0.23 to 12 mM
- Brij L23/35: Detect concentrations ranging from 0.09 to 5 mM
- DTAC: Detect concentrations ranging from 0.08 to 2 mM
- SDS: Detect concentrations ranging from 10 to 25 mM
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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Cet article | |||
|---|---|---|---|
| description Sufficient for 100 colorimetric tests | description Sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description sufficient for 200 colorimetric tests |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| input protein | input plant tissue | input - | input - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition (No metal containers. Tightly closed.) | storage condition - | storage condition - | storage condition - |
| application(s) environmental | application(s) agriculture | application(s) cosmetics | application(s) environmental |
Mot-clé
Danger
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Que se passe-t-il ?
WGK 3
Codes de danger
Mentions de précaution
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1
Classe de stockage
12 - Non Combustible Liquids
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Can the product MAK459 be used with tissue samples?
1 answer-
Helpful?
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Cationic/Nonionic Detergent는 560 nm로 측정하라 돼있는데 590 nm로 측정해도 될까요?
1 answer-
This kit has been optimized for use at 560 nm. It would be up to the end user to determine suitability at a different wavelength.
Helpful?
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