L6669
LuminoCt® qPCR ReadyMix™
For fast probe-based quantitative PCR
Synonyme(s) :
LuminoCt® qPCR ReadyMix™, LuminoCt®
About This Item
Produits recommandés
Forme
liquid
Utilisation
sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions
Caractéristiques
dNTPs included
hotstart
Technique(s)
qPCR: suitable
Couleur
colorless
Entrée
purified DNA
Adéquation
suitable for (quantitative PCR)
Application(s)
agriculture
Compatibilité
Bio-Rad MyiQ ( )
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
Méthode de détection
probe-based
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
- Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
Application
Caractéristiques et avantages
- Ideal for rapid, high-throughput 2-step qPCR protocols
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced preparation time and the risk of contamination from multiple pipetting steps
- Compatible with commonly available real-time instrument platforms for probe-based qPCR assays
- Require small amplicons (<200 bp) for optimal results
- Compatible with commercial primer sets, including TaqMan® assays
- JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation
- Assay results in as little as 25 minutes
- Designed to deliver unsurpassed accuracy, precision, and sensitivity
- Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters
Conditionnement
100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.
Default reaction volume is 50 μL
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Autres remarques
- Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
- At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
- Sigma recommends designing primers and probes with Beacon Designer software
To order primers and probes from Sigma, visit www.sigma.com/oligos
Informations légales
Composants de kit également disponibles séparément
- R4526100X ROX internal reference dyeFDS
Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
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Articles
Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.
Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.
Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.
Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.
Protocoles
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
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