For continuous use, this product should be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/293/677/d9663dat-ms.pdf
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| Taille/Référence | Disponibilité | Prix |
|---|
A propos de cet article
biological source
donkey
Quality Segment
sterility
non-sterile
contains
preservative
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
General description
Application
- comme constituant d'un tampon de blocage pour des analyses par immunofluorescence.
- dans du tampon phosphate salin (PBS) comme agent de blocage.
- comme agent de blocage sur des échantillons congelés analysés en immunocytochimie et immunohistochimie.
- pour prévenir les liaisons non spécifiques durant des analyses d'immunohistochimie sur la parvalbumine.
Disclaimer
1 of 1
Cet article | |||
|---|---|---|---|
| description - | description liquid | description whole antiserum | description whole antiserum |
| biological source donkey | biological source horse serum | biological source rabbit | biological source rabbit |
| sterility non-sterile | sterility non-sterile | sterility - | sterility - |
| application(s) research pathology | application(s) - | application(s) - | application(s) - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe de stockage
10 - Combustible liquids
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Can this product be frozen and thawed without affecting the quality?
1 answer-
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where can I find the protocol to reconstitute this donkey serum
1 answer-
A protocol is not associated with this product. A preservative is added to this serum, making it unsuitable for cell culture. However, in blocking applications, it is typically used at a concentration of 5% - 20%. It may be helpful to view the 'Peer Reviewed Papers' and 'Protocols and Articles' section of the product detail page.
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Is heat inactivation required for D9663 in immunofluorescence (IF)? I found information stating that it is not necessary for immunohistochemistry (IHC). If this applies to IF as well, what is the reason behind it?
1 answer-
Donkey serum does not require heat inactivation for immunofluorescence. It is important to note that this is not a sterile product. While the lab may have routinely heat inactivated their serums for immunofluorescence, this is likely for worker safety rather than a specific requirement for performing immunofluorescence.
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Does donkey serum D9663-10ML require heat inactivation prior to immunohistochemical assay?
1 answer-
There is no need to heat inactivate donkey serum for blocking purposes.
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