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211-500

Endothelial Cell Growth Medium (500 ml)

Synonyme(s) :

Primary Cell Culture Media

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A propos de cet article

NACRES:
NA.71
UNSPSC Code:
12352207
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form

liquid

shelf life

6 mo.

manufacturer/tradename

Cell Applications, Inc

shipped in

wet ice

storage temp.

2-8°C

General description

All-in-one ready-to-use media
Endothelial Cell Growth Medium (500 ml) is an all-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies.

Application

Endothelial Cell Growth Medium (500 ml) has been used to culture human aortic endothelial cells and human umbilical vein endothelial cells.

Preparation Note

contained in protocol

Analysis Note

Each lot was tested for the ability to support plating, spreading and proliferation

Classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. does this medium contain FBS? if so, can u specify the concentration?

    1 answer

    1. This product does contain fetal bovine serum. However, the exact concentration is considered proprietary. Please note that this product does not contain VEGF.

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  2. Does the medium contain VEGF? If so, can you specify the concentration?

    1 answer

    1. This medium does not contain VEGF.

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  3. If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?

    1 answer

    1. This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.

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  4. Can you please provide the full composition of the medium with the concentrations?

    1 answer

    1. This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.

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  5. Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.

    1 answer

    1. This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.

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