NA61
Anti-BrdU (Ab-3) Mouse mAb (Mobu-1)
liquid, clone Mobu-1, Calbiochem®
Synonyme(s) :
Anti-Bromodeoxyuridine
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
Mobu-1, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Réactivité de l'espèce (prédite par homologie)
all
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Description générale
Anti-BrdU (Ab-3), mouse monoclonal, clone Mobu-1, recognizes BrdU. Requires biosynthetic labeling of the target cells with BrdU. It is validated for ICC and use with frozen and paraffin sections.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells (see application references). Recognizes BrdU (bromodeoxyuridine).
Recognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
Immunogène
BrdU-labeled DNA
Application
Frozen Sections (2 g/ml)
Immunocytochemistry (1 g/ml)
Paraffin Sections (2.5 g/ml, trysin pre-treatment required)
Immunoblotting (not recommended)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Remarque sur l'analyse
Negative Control
Unlabeled cells
Unlabeled cells
Positive Control
BrdU labeled DNA
BrdU labeled DNA
Autres remarques
Gratzner, H.G. 1982. Science218, 474.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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