HADCYMAG-61K
MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay
The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.
About This Item
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Milliplex®
assay range
accuracy: 100%
(IL-6)
accuracy: 102%
(TNFα)
accuracy: 103%
(MCP-1)
accuracy: 83%
(PAI-1 (total))
accuracy: 99%
(HGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8)
sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 10 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?)
sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total))
standard curve range: 0.29-4,000 pg/mL
(IL-8)
standard curve range: 0.32-5,000 pg/mL
(NGF)
standard curve range: 0.38-6,000 pg/mL
(TNFα)
standard curve range: 0.51-8,000 pg/mL
(IL-1β)
standard curve range: 0.96-150,000 pg/mL
(IL-6)
standard curve range: 0.96-150,000 pg/mL
(MCP-1)
standard curve range: 26-400,000 pg/mL
(Adipnectin)
standard curve range: 6.4-100,000 pg/mL
(HGF)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Resistin)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
inter-assay cv: <20%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <20%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total))
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β)
inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)
Technique(s)
multiplexing: suitable
Méthode de détection
fluorometric (Luminex xMAP)
Conditions d'expédition
wet ice
Description générale
The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Spécificité
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Application
- Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
- Recommended Sample Type: Tissue/cell culture supernatants or extracts
- Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine
Caractéristiques et avantages
Conditionnement
Stockage et stabilité
Autres remarques
Informations légales
Clause de non-responsabilité
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificats d'analyse (COA)
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Contenu apparenté
Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
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