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SRP3147

Sigma-Aldrich

Relaxin-2 Peptide

≥98% (HPLC), human recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

H2 relaxin, RLN2

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

product name

Relaxin-2 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

6.0 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RLN2(6019)

General description

Relaxin-2 is a peptide hormone structurally related to insulin. The gene is mapped to human chromosome 9p24. It is expressed in the placenta, decidua, prostate, corpus luteum, endometrium, mammary glands, heart, and brain. It is also expressed in the ovary during pregnancy. Recombinant relaxin-2 is a nonglycosylated 6kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.

Application

It has also been used to study its effect on human endometrial carcinoma cell lines.
Relaxin-2 human has been used to study the effect of testosterone on the expression of relaxin receptor in the knee joint.

Biochem/physiol Actions

Of the three known relaxin genes, Relaxin-2 (RLN2) is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 (leucine-rich repeat-containing G-protein coupled receptor 7) and LGR8 receptors, previously identified as an "orphan" G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Relaxin-2 also participates in cancer progression. In prostate cancer, it is responsible for tumor growth and vascularisation. Relaxin-2 is also linked with anti-fibrotic and cardioprotective effects.

Sequence

A-Chain: QLYSALANKC CHVGCTKRSL ARFC B-Chain: DSWMEEVIKL CGRELVRAQI AICGMSTWS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evolution of the relaxin/insulin-like gene family in anthropoid primates.
Arroyo JI, et al.
Genome Biology and Evolution, 6, 491-499 (2014)
The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1.
Sethi A, et al.
Nature Communications, 7, 11344-11344 (2016)
Testosterone reduces knee passive range of motion and expression of relaxin receptor isoforms via 5a-dihydrotestosterone and androgen receptor binding.
Dehghan F, et al.
International Journal of Molecular Sciences, 15, 4619-4634 (2014)
Relaxin as a cardiovascular hormone: physiology, pathophysiology and therapeutic promises.
Nistri S, et al.
Cardiovascular & Hematological Agents in Medicinal Chemistry, 5, 101-108 (2007)
Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the beta-Catenin Pathway in Endometrial Cancer
Fue M, et al.
International Journal of Molecular Sciences, 19(8), 2438-2438 (2018)

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