Skip to Content
Merck
All Photos(1)

Documents

L2769

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Phaseolus vulgaris

Quality Level

200

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.)
≤1.0 μg/mL leucoagglutination activity
≤5 μg/mL mitogenic activity

composition

Protein, ≥60% biuret

impurities

salt, free

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • as a positive control in ELISPOT (enzyme-linked immunospot) assay
  • to induce Th2 polarization for T-cell differentiation in vitro
  • as controls in the lymphocyte proliferation assays

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Preparation Note

Purified by a modification of the method of Leavitt.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Linc-MAF-4 regulates Th1/Th2 differentiation and is associated with the pathogenesis of multiple sclerosis by targeting MAF
Zhang F, et al.
Faseb Journal, 31(2), 519-525 (2016)
Variation in gamma interferon responses to different infecting strains of Mycobacterium tuberculosis in acid-fast bacillus smear-positive patients and household contacts in Antananarivo, Madagascar
Rakotosamimanana N, et al.
Clinical and Vaccine Immunology : CVI, 17(7), 1094-1103 (2010)
Fan Yang et al.
Gene therapy, 29(1-2), 41-54 (2021-01-13)
Adeno-Associated Virus (AAV)-based gene therapy vectors are in development for many inherited human disorders. In nonclinical studies, cellular immune responses mediated by cytotoxic T cells may target vector-transduced cells, which could impact safety and efficacy. Here, we describe the bioanalytical
Carla M S Ribeiro et al.
Nature, 540(7633), 448-452 (2016-12-06)
The most prevalent route of HIV-1 infection is across mucosal tissues after sexual contact. Langerhans cells (LCs) belong to the subset of dendritic cells (DCs) that line the mucosal epithelia of vagina and foreskin and have the ability to sense
K J MacLeod et al.
Hormones and behavior, 136, 105072-105072 (2021-10-11)
Reproduction is a critical part of an animal's life history, but one which incurs significant costs to survival and future reproductive potential. These physiological consequences are likely to be influenced by context - for example, if an individual is subject
View All Related Papers

Articles

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service