ZRB2271

Anti-SMAD7 Antibody, clone 2D15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Anti-IL-13 Antibody, clone 1B10 ZooMAb^® Rabbit Monoclonal, MAD homolog 7, MAD homolog 8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, SMAD family member 7, hSMAD7
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2D15, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 46.43 kDa; observed mol wt ~40 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse, rat
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_005895
• Numéro d'accès UniProt: O15105
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... SMAD7(4092)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 2D15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects SMAD7. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SMAD7.

Application

Quality Control Testing

Evaluated by Western Blotting in SH-SY6Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SMAD7 in SH-SY5Y cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SMAD7 in A549 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SMAD7 in U-251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound SMAD7 with a KD of 1.2 x 10^-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SMAD7 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Mothers against decapentaplegic homolog 7 (UniProt: O15105; also known as MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, Smad7, hSMAD7) is encoded by the SMAD7 (also known as MADH7, MADH8) gene (Gene ID: 4092) in human. At least nine SMAD proteins have been described in vertebrates that are classified as: (a) receptor-activated SMADs (R-SMADs), (b) co-mediator SMADs, and (c) inhibitory SMADs (I-SMADs). SMAD6 and 7 belong to the group of I-SMADs. SMAD7 is ubiquitously expressed protein with higher expression observed in the lung and vascular endothelium. It is a key negative regulator of TGF-β signaling and plays an important role in modulating a large array of biological processes. It antagonizes TGF-β signaling through multiple mechanisms, such as by interfering with the recruitment of R-SMADs upon TGF-β/activin activation, by inducing degradation of TGF-β type I r

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable