Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

Y1003

Sigma-Aldrich

2x YT medium

Liquid microbial growth medium

Synonyme(s) :

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41106225
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Stérilité

sterile; 0.2 μm filtered

Forme

liquid

Technique(s)

microbiological culture: suitable

pH

6.5-7.5

Application(s)

microbiology

Température de stockage

room temp

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Catégories apparentées

Description générale

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Caractéristiques et avantages

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Composants

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 5

1 of 5

Bouillon LB (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

Bouillon LB (Lennox)

Terrific broth suitable for microbiology

Millipore

1.01629

Terrific broth

Tryptone Microbiologically tested.

Millipore

T7293

Tryptone

CelLytic™ B Cell Lysis Reagent For bacterial cell lysis, 10× concentrate

Sigma-Aldrich

C8740

CelLytic™ B Cell Lysis Reagent

Sigma-Aldrich

Sigma-Aldrich

M6030

M9, Minimal Salts, 5X

Dynamical model fitting to a synthetic positive feedback circuit in E. coli
Tica J. et al.
Journal of Separation Science (2020)
Victor V Kuprianov et al.
Virus research, 284, 197984-197984 (2020-04-24)
Hepatitis C virus (HCV) can cause chronic infection and evade the immune response. The generation and maintenance of an effective T-cell response is important for immune-mediated control of HCV infection. The purpose of this study was to obtain recombinant mosaic
Nicholas Collins et al.
Cell, 178(5), 1088-1101 (2019-08-24)
Mammals evolved in the face of fluctuating food availability. How the immune system adapts to transient nutritional stress remains poorly understood. Here, we show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but
Hong-Yi Kuo et al.
Glycobiology, 26(2), 155-165 (2015-10-09)
Galectin-3 is a ubiquitous lectin exerting multiple cellular functions such as RNA splicing, protein trafficking and apoptosis. Its expression is positively correlated with the poor prognosis in lung cancer patients. Galectin-3 can promote cancer progression through its effects on cell
Zehua Sun et al.
Frontiers in immunology, 13, 869825-869825 (2022-04-26)
Phage display is a well-established technology for in vitro selection of monoclonal antibodies (mAb), and more than 12 antibodies isolated from phage displayed libraries of different formats have been approved for therapy. We have constructed a large size (10^11) human
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique