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U0880

Sigma-Aldrich

Uricase from Candida sp.

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonyme(s) :

Urate: oxygen oxoreductase

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About This Item

Numéro CAS:
9002-12-4
Numéro de classification (Commission des enzymes):
1.7.3.3 (BRENDA, IUBMB)
Numéro MDL:
MFCD00082126
Code UNSPSC :
12352204
ID de substance PubChem :
24900619
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

≥2 units/mg solid

Poids mol.

35 kDa by SDS-PAGE

Température de stockage

−20°C

Chaîne SMILES 

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

Clé InChI

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description générale

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Actions biochimiques/physiologiques

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Définition de l'unité

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Forme physique

Powder; contains citrate and sucrose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000380939
0000349793
0000327059
0000380939
0000327059

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Journal of the American Society of Nephrology : JASN, 12(5), 1001-1009 (2001-04-24)
Uricase-deficient mice develop uric acid nephropathy, with high mortality rates before weaning. Urate excretion was quantitated and renal function was better defined in this study, to facilitate the use of these mice as a model for evaluating poly(ethylene glycol)-modified recombinant
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By using red wine, dealcoholized red wine, polyphenols-stripped red wine, ethanol-water solution and water, the role of wine polyphenols and induction of plasma urate elevation on plasma antioxidant capacity was examined in humans (n=9 per beverage). Healthy males randomly consumed
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Questions

1–2 of 2 Questions  

  1. How can a solution in unit/mL be prepared using product number U0880, with a pack size of 250 units and SKU U0880-250UN, considering that the uricase activity is greater than 2 units/mg solid? What is the procedure for preparing a 10 unit/mL solution from this?

    1 answer

    1. You can use the calculator provided at https://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html

      Based on a specific activity of 5 units/mg, the 250 unit pack size is equivalent to 50 mg in mass. To achieve a 10 units/mL solution from this, the 50 mg would need to be dissolved in 25 mL of solvent (e.g. PBS).

      Helpful?

  2. What is the amino acid sequence of this enzyme?

    1 answer

    1. The sequence is proprietary.

      Helpful?

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