T8944
Topoisomerase II α human
(Single band on SDS-PAGE), liquid
Synonyme(s) :
Topoisomerase II alpha, TOPO® II, p170
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About This Item
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Niveau de qualité
Forme
liquid
Poids mol.
170 kDa
Numéro d'accès UniProt
Température de stockage
−70°C
Informations sur le gène
human ... TOP2A(7153)
Description générale
Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
Topoisomerase II α is encoded by TOP2A gene, which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication, recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer, ovarian cancer and prostate cancer.
Application
Human topoisomerase II α has been used in DNA cleavage assay.
Topoisomerase II α has been used in a study to assess aging processes in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication.
Actions biochimiques/physiologiques
Used to study DNA structure and topology.
Définition de l'unité
One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.
Forme physique
Buffered aqueous glycerol solution, containing Trizma buffer (pH 7.7). Other components are present in the solution, and lot-specific information on these components may be obtained from Technical Service.
Notes préparatoires
Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.
Informations légales
TOPO is a registered trademark of Life Technologies
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Raj Kumar Mandraju et al.
Archives of biochemistry and biophysics, 470(1), 27-34 (2007-11-21)
Topoisomerase II alpha (TopoIIalpha) and Topoisomerase II beta (TopoIIbeta) isoforms are different gene products having conserved catalytic activities. The alpha isoform is present in proliferating cell, while beta isoform is predominantly present in non-proliferating cells namely neurons suggesting its role
The prognostic significance of topoisomerase II alpha protein in early stage luminal breast cancer.
An X, et al.
BMC Cancer, 18(1), 331-331 (2018)
Topoisomerase 2 alpha: a real predictor of anthracycline efficacy?
Romero A, et al.
Clinical And Translational Oncology, 14(3), 163-168 (2012)
Yoshihiko Ichikawa et al.
The Journal of clinical investigation, 124(2), 617-630 (2014-01-03)
Doxorubicin is an effective anticancer drug with known cardiotoxic side effects. It has been hypothesized that doxorubicin-dependent cardiotoxicity occurs through ROS production and possibly cellular iron accumulation. Here, we found that cardiotoxicity develops through the preferential accumulation of iron inside
J R Spitzner et al.
Nucleic acids research, 16(12), 5533-5556 (1988-06-24)
Topoisomerase II, purified from chicken erythrocytes, was reacted with a large number of different DNA fragments and cleavages were catalogued in the presence and absence of drugs that stabilize the cleavage intermediate. Cleavages were sequenced to derive a consensus for
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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