SRP8024

TRAIL, Soluble human

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

• Synonyme(s) : Apo-2L, CD253, CD339, TRAIL
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Étiquette/Marqueur: His tagged
• Pureté: >98% (SDS-PAGE)
• Forme: liquid
• Poids mol.: ~26 kDa
• Conditionnement: pkg of 20 μg
• Concentration: ≥0.2 mg/mL
• Technique(s): activity assay: suitable; cell culture | mammalian: suitable; transfection: suitable
• Impuretés: <0.01 EU/μg endotoxin, tested
• Couleur: clear
• Numéro d'accès UniProt: P50591
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... TNFSF10(8743)

Description

Description générale

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Actions biochimiques/physiologiques

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Forme physique

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Autres remarques

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable