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Key Documents

SRP3234

Sigma-Aldrich

SCF from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonyme(s) :

Mast Cell Growth Factor (MGF), Steel Factor, Stem Cell Factor, c-Kit Ligand

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

mouse

Produit recombinant

expressed in E. coli

Pureté

≥98% (HPLC)
≥98% (SDS-PAGE)

Forme

lyophilized

Puissance

≤10.0 ng/mL ED50

Poids mol.

18.3 kDa

Conditionnement

pkg of 10 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white

Numéro d'accès UniProt

P20826

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... KITL(17311)

Description générale

Stem cell factor (SCF), which is also known as kit ligand (KITL), is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.3kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Actions biochimiques/physiologiques

SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. In mice, this protein has been shown to improve the development of ovarian follicles.

Séquence

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Forme physique

Lyophilized from 10 mM Sodium Phosphate, pH 6.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Edyta E Wojtowicz et al.
Experimental hematology, 42(10), 909-918 (2014-08-06)
MicroRNAs (miRNAs) are crucial for proper functioning of hematopoietic stem and progenitor cells (HSPCs). Members of the miRNA-125 family (consisting of miR-125a, miR-125b1, and miR-125b2) are known to confer a proliferative advantage on cells upon overexpression, to decrease the rate
Laura Pitzonka et al.
PloS one, 9(5), e97628-e97628 (2014-05-17)
Co-transcriptionally assembled ribonucleoprotein (RNP) complexes are critical for RNA processing and nuclear export. RNPs have been hypothesized to contribute to the regulation of coordinated gene expression, and defects in RNP biogenesis contribute to genome instability and disease. Despite the large
Kit ligand decreases the incidence of apoptosis in cultured vitrified whole mouse ovaries.
Abdi S
Reproductive Biomedicine Online, 30(5), 493-503 (2015)
D M Anderson et al.
Cell, 63(1), 235-243 (1990-10-05)
We have previously reported the identification of a novel mast cell growth factor (MGF) that was shown to be a ligand for c-kit and is encoded by a gene that maps near the steel locus on mouse chromosome 10. We
T R Ulich et al.
Blood, 78(3), 645-650 (1991-08-01)
Recombinant rat stem cell factor (rrSCF) administered to rats as a single intravenous injection causes a dose-dependent neutrophilia and lymphocytosis as well as the appearance of immature myeloid cells and occasional blast cells in the circulation. Neutrophilia begins at 2
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