This product only contains 5 micrograms of protein without any carrier protein. The product is lyophilized from a small amount of solution so the product may not be visible in the vial.
G5035
Granulocyte-Macrophage Colony-Stimulating Factor human
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonyme(s) :
GM-CSF human
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Nom du produit
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Essai
≥98% (SDS-PAGE)
Forme
lyophilized powder
Puissance
≤0.1 ng/mL EC50
Poids mol.
14.6 kDa
Conditionnement
pkg of 4X5 μg
pkg of 5 μg
Conditions de stockage
avoid repeated freeze/thaw cycles
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1.000 EU/μg
Couleur
white
Solubilité
water: soluble 0.010 mL, clear, colorless
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... CSF2(1437) , CSF2(1437)
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Package size based on protein content
Actions biochimiques/physiologiques
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Forme physique
Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.
Remarque sur l'analyse
The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.[1]
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Alessio Lanna et al.
Methods in molecular biology (Clifton, N.J.), 2654, 251-261 (2023-04-28)
Eukaryotes solve the DNA-end replication problem synthesizing hexameric chromosome ends known as telomeres. Recent studies have uncovered unexpected functions of telomeres in linking synaptic signaling and vesicle transport, with at least one pathway directly involved in transferring telomeres through the
Benhur Lee et al.
Research square (2021-10-07)
Bats are significant reservoir hosts for many viruses with zoonotic potential1. SARS-CoV-2, Ebola virus, and Nipah virus are examples of such viruses that have caused deadly epidemics and pandemics when spilled over from bats into human and animal populations2,3. Careful
Satoshi Ikegame et al.
Nature microbiology, 8(6), 1108-1122 (2023-05-05)
Morbilliviruses are among the most contagious viral pathogens of mammals. Although previous metagenomic surveys have identified morbillivirus sequences in bats, full-length morbilliviruses from bats are limited. Here we characterize the myotis bat morbillivirus (MBaMV) from a bat surveillance programme in
Rongqing Pan et al.
Cell, 185(9), 1521-1538 (2022-04-22)
Interest in harnessing natural killer (NK) cells for cancer immunotherapy is rapidly growing. However, efficacy of NK cell-based immunotherapy remains limited in most trials. Strategies to augment the killing efficacy of NK cells are thus much needed. In the current
Antonella Mancusi et al.
Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in
Articles
Read article on hematopoietic cytokines and hematopoiesis
-
There is no visible powder in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human. Is the vial empty?
1 answer-
Helpful?
-
-
Can I use Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human, on mouse cells?
1 answer-
There is only about 56% homology between human and mouse GM-CSF, and no cross-species activity. We would recommend to use mouse GM-CSF, Product No. G0282.
Helpful?
-
-
What is the isoelectric point (pI) for Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 answer-
The pI is 4.8.
Helpful?
-
-
How many units are present in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 answer-
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of greater than or equal to 1 x 107 units/mg. So the 5 microgram package size (G5035-5UG) should have at least 50,000 units per vial.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
