SRE0045

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, lyophilized powder, ≥10×10¹⁰ units/mg protein

• Synonyme(s) : Luciferase firefly
• Numéro CAS: 61970-00-1
• Numéro de classification (Commission des enzymes): 1.13.12.7 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131546
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥10×10¹⁰ units/mg protein
• Poids mol.: 62 kDa
• Application(s): diagnostic assay manufacturing
• Température de stockage: −20°C

Description

Application

Firefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function.

Actions biochimiques/physiologiques

Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light.

Définition de l'unité

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of ¹⁴C in a PPO/POPOP cocktail.

Forme physique

Supplied as a lyophilized powder containing HEPES, pH 7.5, NaCl, MgCl₂, EDTA, DTT and a carbohydrate stabilizer.

This product is a recombinant luciferase (62 kDa) from Photinus pyralis (American firefly) produced from the luc gene expressed in E. coli.

Notes préparatoires

To obtain maximal solubility it is important to reconstitute the enzyme at a high salt concentration, such as 1 M Tris buffer with any counter ion at pH 7-8. The enzyme can be prepared at a concentration of up to 5 mg protein/ml. Do not vortex and avoid agitation.
After reconstitution, the enzyme solutions can kept at 4-8 °C for up to 2 days or frozen in working aliquots at -20°C for at least one month. Repeated freezing and thawing is not recommended.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318,H334
• Conseils de prudence: P261 - P280 - P284 - P305 + P351 + P338 - P501
• Classification des risques: Eye Dam. 1 - Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable