Accéder au contenu
Merck
Toutes les photos(3)

Key Documents

View All Documentation

SAB4200661

Sigma-Aldrich

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse

clone DYN2-11, purified from hybridoma cell culture

Synonyme(s) :

CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin 2

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(3)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

DYN2-11

Forme

buffered aqueous solution

Poids mol.

antigen ~98 kDa

Espèces réactives

mouse, human, monkey, canine, rat

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells.
immunofluorescence: 5-10 μg/mL using using HeLa cells.
immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells.

Isotype

IgG1

Numéro d'accès UniProt

P50570

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DNM2(1785)
mouse ... Dnm2(13430)
rat ... Dnm2(25751)

Description générale

Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins.
The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD).

Immunogène

synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH

Application

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Actions biochimiques/physiologiques

DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer.
Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Riad Efendiev et al.
The Journal of biological chemistry, 277(46), 44108-44114 (2002-09-03)
Clathrin-dependent endocytosis of Na(+),K(+)-ATPase in response to dopamine regulates its catalytic activity in intact cells. Because fission of clathrin-coated pits requires dynamin, we examined the mechanisms by which dopamine receptor signals promote dynamin-2 recruitment and assembly at the site of
Gonçalo C Pereira et al.
PloS one, 15(6), e0234653-e0234653 (2020-06-25)
We previously demonstrated that hexokinase II (HK2) dissociation from mitochondria during cardiac ischemia correlates with cytochrome c (cyt-c) loss, oxidative stress and subsequent reperfusion injury. However, whether HK2 release is the primary signal mediating this ischemia-induced mitochondrial dysfunction was not
Gina L Razidlo et al.
Developmental cell, 24(6), 573-585 (2013-03-30)
The large GTPase Dynamin 2 (Dyn2) is markedly upregulated in pancreatic cancer, is a potent activator of metastatic migration, and is required for Rac1-mediated formation of lamellipodia. Here we demonstrate an unexpected mechanism of Dyn2 action in these contexts via
Bin Xu et al.
Cancer medicine, 3(1), 14-24 (2014-01-10)
Dynamin 2 (Dyn2) is essential for intracellular vesicle formation and trafficking, cytokinesis, and receptor endocytosis. In this study, we investigated the implication of Dyn2 as a prognostic marker and therapeutic target for progressive prostate cancer (PCA). We evaluated Dyn2 protein
Marc Bitoun et al.
Nature genetics, 37(11), 1207-1209 (2005-10-18)
Autosomal dominant centronuclear myopathy is a rare congenital myopathy characterized by delayed motor milestones and muscular weakness. In 11 families affected by centronuclear myopathy, we identified recurrent and de novo missense mutations in the gene dynamin 2 (DNM2, 19p13.2), which
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique