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SAB4200094

Sigma-Aldrich

Anti-PKM2 (isoform M1) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-CTHBP, Anti-OIP3 (OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~60 kDa

Espèces réactives

human, rat, mouse

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.5 mg/mL

Technique(s)

immunocytochemistry: 5-10 μg/mL using HeLa cells
immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon
immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction)
western blot: 1-2 μg/mL using mouse brain extract (S2 fraction)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PKM2(5315)

Catégories apparentées

Description générale

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. The M2 isoform has been linked to cancer metastasis. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues.

Spécificité

Anti-PKM2 (isoform M1) antibody is specific for human, mouse and rat PKM2 (Isoform M1)/PKM1. In immunoblotting, detection of the PKM2 (Isoform M1) /PKM1 band is specifically inhibited by the immunizing peptide.

Application

Anti-PKM2 (isoform M1) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry
  • immunocytochemistry

Actions biochimiques/physiologiques

Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Ataxin-1 regulates the cerebellar bioenergetics proteome through the GSK3beta-mTOR pathway which is altered in Spinocerebellar ataxia type 1 (SCA1)
Sanchez I, et al.
Human Molecular Genetics, 25(18), 4021-4040 (2016)
Mohammed Alquraishi et al.
Cell communication and signaling : CCS, 20(1), 76-76 (2022-06-01)
Acute kidney injury (AKI) is associated with a severe decline in kidney function caused by abnormalities within the podocytes' glomerular matrix. Recently, AKI has been linked to alterations in glycolysis and the activity of glycolytic enzymes, including pyruvate kinase M2
Zhitao Chen et al.
Oncology reports, 50(5) (2023-09-21)
In the present study, it was aimed to investigate the effects and potential mechanisms of heat shock protein B7 (HSPB7) on lung adenocarcinoma (LUAD). Bioinformatic analysis was performed to explore the association between HSPB7 expression and patients with LUAD. MTT
Seung-Yon Lee et al.
The Journal of clinical investigation, 128(12), 5573-5586 (2018-10-05)
Notch signaling critically controls cell fate decisions in mammals, both during embryogenesis and in adults. In the skeleton, Notch suppresses osteoblast differentiation and sustains bone marrow mesenchymal progenitors during postnatal life. Stabilizing mutations of Notch2 cause Hajdu-Cheney syndrome, which is
Jianshuang Guo et al.
Frontiers in oncology, 9, 993-993 (2019-10-22)
Glioblastoma (GBM) is the most prevalent malignant tumor in the central nervous system. Aerobic glycolysis, featured with elevated glucose consumption and lactate production, confers selective advantages on GBM by utilizing nutrients to support rapid cell proliferation and tumor growth. Pyruvate
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