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S7571

Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

greener alternative

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

Synonyme(s) :

CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase

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About This Item

Numéro CAS:
9054-89-1
Numéro de classification (Commission des enzymes):
1.15.1.1 (BRENDA, IUBMB)
Numéro CE :
232-943-0
Numéro MDL:
MFCD00132404
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bovine

Forme

lyophilized powder

Activité spécifique

≥3,000 units/mg protein

Poids mol.

32.5 kDa

Composition

Protein, ≥95% biuret

Conditions de stockage

(Store under nitrogen.
Tightly closed. Dry.)

Caractéristiques du produit alternatif plus écologique

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

immunoblotting: suitable
inhibition assay: suitable

Couleur

blue-green

pl 

4.95

Solubilité

water: 20 mg/mL
aqueous buffer, pH 7.5: soluble

Numéro d'accès UniProt

P00442
P41976

Application(s)

diagnostic assay manufacturing

Autre catégorie plus écologique

, Re-engineered

Température de stockage

−20°C

Informations sur le gène

cow ... SOD1(281495) , SOD2(281496)

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Catégories apparentées

Description générale

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

Application

Superoxide dismutase from bovine erythrocytes has been used:

  • in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
  • in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
  • to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
  • in combination with catalase to study its effect on cell differentiation in vitro
  • to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Actions biochimiques/physiologiques

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Définition de l'unité

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Forme physique

Lyophilized powder, essentially salt-free

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Michael A Hough et al.
Structure (London, England : 1993), 11(8), 937-946 (2003-08-09)
Copper zinc superoxide dismutase (CuZnSOD) forms a crucial component of the cellular response to oxidative stress by catalyzing the dismutation of the superoxide radical to hydrogen peroxide and water. Mutations in human CuZnSOD are associated with the development of familial
Possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction.
F Hirata et al.
The Journal of biological chemistry, 246(24), 7825-7826 (1971-12-25)
Sebastian Overmans et al.
Aquatic toxicology (Amsterdam, Netherlands), 198, 165-174 (2018-03-20)
Exposure to polycyclic aromatic carbons (PAHs) poses a growing risk to coral reefs due to increasing shipping and petroleum extraction in tropical waters. Damaging effects of specific PAHs can be further enhanced by the presence of ultraviolet radiation, known as
Superoxide dismutases and superoxide reductases.
Yuewei Sheng et al.
Chemical reviews, 114(7), 3854-3918 (2014-04-02)
Colleen M Hansel et al.
mBio, 10(2) (2019-03-14)
There is a growing appreciation within animal and plant physiology that the reactive oxygen species (ROS) superoxide is not only detrimental but also essential for life. Yet, despite widespread production of extracellular superoxide by healthy bacteria and phytoplankton, this molecule
Afficher tous les articles scientifiques apparentés

Articles

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Protocoles

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

Separation of Superoxide dismutase

Chromatograms

application for HPLC

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