S6510

N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin

≥90% (HPLC)

• Formule empirique (notation de Hill): C₄₀H₅₃N₅O₁₀
• Numéro CAS: 94367-21-2
• Poids moléculaire : 763.88
• Numéro MDL: MFCD00080248
• Code UNSPSC : 12352200
• ID de substance PubChem : 24899709
• Nomenclature NACRES : NA.77

Propriétés

• Pureté: ≥90% (HPLC)
• Forme: powder
• Solubilité: 0.1% trifluoroacetic acid in acetonitrile: water (3:1): 1 mg/mL, clear, colorless
• Température de stockage: −20°C
• Chaîne SMILES: CC(C)CC(NC(=O)CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(Cc1ccc(O)cc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2
• InChI: 1S/C40H53N5O10/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49)
• Clé InChI: UVFAEQZFLBGVRM-UHFFFAOYSA-N

Description

Amino Acid Sequence

N-Suc-Leu-Leu-Val-Tyr-7-AMC

Application

N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin was used in proteasome chymotrypsin-like activity assay in Jurkat cell lysate⁶ and crude cell lysate of rice.⁷

Actions biochimiques/physiologiques

In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.⁶

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrats

Fluorogenic substrate for chymotrypsin-like enzymes, such as cathepsin B and calpain which have been implicated in programmed cell death.

Informations sur la sécurité

• Mentions de danger: H413
• Conseils de prudence: P273
• Classification des risques: Aquatic Chronic 4
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)