S0692

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1

• Synonyme(s) : Actinospectacin
• Numéro MDL: MFCD04041129
• Code UNSPSC : 51281602
• Nomenclature NACRES : NA.85

Propriétés

• Source biologique: Streptomyces sp.
• Niveau de qualité: 200
• Forme: solution
• Concentration: 100 mg/mL in DMSO/H₂O, 1:1
• Couleur: colorless
• Solubilité: DMSO/H₂O, 1:1: soluble 100 mg/mL
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• Mode d’action: protein synthesis | interferes
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Chemical structure: aminoglycoside

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Actions biochimiques/physiologiques

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Attention

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Notes préparatoires

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

Stockage et stabilité

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 201.0 °F - closed cup
• Point d'éclair (°C): 93.9 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)