RAB0477

Mouse Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

• Synonyme(s) : Cachectin, TNF-alpha, Tumor necrosis factor alpha
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Tnf(21926)
• Input: sample type serum; sample type cell culture supernatant(s); sample type plasma
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Propriétés

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type serum; sample type cell culture supernatant(s); sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 60 pg/mL; standard curve range: 93.75-6000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Tnf(21926)

Description

General description

Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse TNF-α

Application

Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.

Biochem/physiol Actions

Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

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  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

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• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

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• RABSTOP3

  ELISA Stop Solution (Item I)

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• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

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• RABWASH4

  20X Wash Buffer (Item B)

  FDS

Informations sur la sécurité

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Classe de stockage: 10 - Combustible liquids