MAK518

ADP Assay Kit

sufficient for 100 fluorometric tests

• Synonyme(s) : ADP Detection Kit
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): hematological disorder
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.

Caractéristiques et avantages

• As low as 0.1 µM ADP can be quantified.
• Compatible with high-throughput handling systems.

Adéquation

The kit is suitable for ADP determination in cells and other biological samples.

Principe

The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H290,H314,H335,H411
• Conseils de prudence: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2