MAK107
LCAT Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Synonyme(s) :
Lecithin:cholesterol acyltransferase activity assay kit
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About This Item
Produits recommandés
Utilisation
sufficient for 100 fluorometric tests
Application(s)
pharmaceutical
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
cardiovascular diseases
Température de stockage
−20°C
Informations sur le gène
human ... LCAT(3931)
Description générale
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.
Application
Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for measuring phospholipase activity of LCAT in plasma or serum.
Principe
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.
Remarque sur l'analyse
Composants de kit seuls
Réf. du produit
Description
- Substrate Reagent .1 mL
- READ Reagent 30 mL
- LCAT Assay Buffer 20 mL
Produit(s) apparenté(s)
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
49.5 °F
Point d'éclair (°C)
9.7 °C
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