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L7025

Sigma-Aldrich

LB Broth with agar (Lennox)

Tablet microbial growth medium

Synonyme(s) :

LB Agar, LB agar

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

for molecular biology

Niveau de qualité

200

Stérilité

non-sterile

Forme

tablet

Technique(s)

microbiological culture: suitable

Application(s)

food and beverages
microbiology

Température de stockage

room temp

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Catégories apparentées

Description générale

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caractéristiques et avantages

Lennox LB tablet with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Composants

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Reconstitution

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Informations légales

Zeocin is a registered trademark of Cayla Sarl

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

L7275

LB Broth (Lennox), Tablet microbial growth medium

L7658

LB Broth (Lennox), EZMix powder microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix powder microbial growth medium

L3022

Bouillon LB (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

Bouillon LB avec gélose (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000378729
0000378730
0000304802
0000304796
0000304797

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Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
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Protocoles

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Questions

1–5 of 5 Questions  

  1. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  2. Will adding magnesium to the culture medium increase cell density?

    1 answer

    1. In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator.

      Helpful?

  3. What is the difference among the many LB - Lennox products?

    1 answer

    1. LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix™ powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    1 answer

    1. LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version).

      Helpful?

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