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H8280

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

Synonyme(s) :

N-Hydroxysuccinimidyl-Agarose

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A propos de cet article

NACRES:
NA.56
UNSPSC Code:
23151817
MDL number:
MFCD00164950
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form

isopropanol suspension

Quality Segment

200

extent of labeling

16-23 μmol per mL

technique(s)

protein array: suitable

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

General description

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

  • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation, which gives a high degree of substitution of the selected ligand
  • NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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