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E2412

Sigma-Aldrich

Pullulanase microbial

Synonyme(s) :

Pullulanase microbial, Promozyme® D2

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About This Item

Numéro CAS:
9075-68-7
Numéro de classification (Commission des enzymes):
3.2.1.41 (BRENDA, IUBMB)
Numéro MDL:
MFCD00081941
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Produit recombinant

expressed in Bacillus subtilis

Niveau de qualité

200

Forme

aqueous solution

Activité spécifique

≥1000 NPUN/g

Température de stockage

2-8°C

Application

Pullulanase is a glucanase that degrades pullulan. It is commonly used for starch-debranching as well as to study polysaccharide utilization[1].

Actions biochimiques/physiologiques

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations légales

A product of Novozymes Corp.
Novozym is a registered trademark of Novozymes A/S
Promozyme is a trademark of Novozymes Corp.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000373134
0000258717
0000305950
0000305951
0000373134

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Nasima Chorfa et al.
Polymers, 14(10) (2022-05-29)
In this work, a valorization of the starch stemming from downgraded potatoes was approached through the preparation of starch nanoparticles using different physical methods, namely liquid and supercritical carbon dioxide, high energy ball milling (HEBM), and ultrasonication on the one
Christopher C Ibenegbu et al.
Microbial cell factories, 21(1), 251-251 (2022-11-30)
The starch in waste bread (WB) from industrial sandwich production was directly converted to ethanol by an amylolytic, ethanologenic thermophile (Parageobacillus thermoglucosidasius strain TM333) under 5 different simultaneous saccharification and fermentation (SSF) regimes. Crude α-amylase from TM333 was used alone
K A Smith et al.
Journal of bacteriology, 171(4), 2116-2123 (1989-04-01)
We have cloned a pullulanase gene from Bacteroides thetaiotaomicron. The pullulanase expressed from this clone in Escherichia coli was cell associated and soluble and had a molecular mass of 72 kilodaltons by gel filtration. Maxicell analysis of proteins coded by
Johanne Mørch Jensen et al.
Protein expression and purification, 79(2), 217-222 (2011-05-05)
The limit dextrinase inhibitor (LDI) from barley seeds acts specifically on limit dextrinase (LD), an endogenous starch debranching enzyme. LDI is a 14 kDa hydrophobic protein containing four disulfide bonds and one unpaired thiol group previously found to be either
Malene Bech Vester-Christensen et al.
Journal of molecular biology, 403(5), 739-750 (2010-09-25)
Barley limit dextrinase [Hordeum vulgare limit dextrinase (HvLD)] catalyzes the hydrolysis of α-1,6 glucosidic linkages in limit dextrins. This activity plays a role in starch degradation during germination and presumably in starch biosynthesis during grain filling. The crystal structures of
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Questions

1–3 of 3 Questions  

  1. May I know how to make 10units/mL sample from the bottle?

    1 answer

    1. The activity in the batch-specific Certificate of Analysis is expressed as NPUN/g. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN, where 1 PUN is defined as the amount of enzyme that releases 1 μmol of glucose per minute under standard conditions.

      The Certificate of Analysis also reports the density (g/mL) of the product. To calculate the activity in units per milliliter (UPUN/mL), multiply the activity (NPUN/g) by the density (g/mL). Further dilution can then be performed to achieve the desired final concentration.

      Helpful?

  2. I was wondering if product E2412-50ML is a type 1 or type 2 pullulanase (degrades alpha1,6 linkages only or both alpha1,4 and 1,6?)

    1 answer

    1. E2412 contains both Type 1 and Type 2 Pullulanases.

      Helpful?

  3. What is the molecular weight of this Pullulanase enzyme? And is there any glucose in the enzyme solution?

    1 answer

    1. The molecular weight has not been determined, however, a search of the literature indicates that the general molecular weight of Pullulanase, CAS 9075-68-7, ranges from 70 - 115 kDa. This is a product of Novozymes Corp. The manufacturer considers the formulation of this product proprietary but has noted that this material is pure Oligo-1,6-Glucosidase.

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