D5030

Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : DME, DMEM
• NACRES: NA.75
• UNSPSC Code: 12352207

Propriétés

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: phenol red: no; NaHCO₃: no; L-glutamine: no; HEPES: no; glucose: no; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Application

Dulbecco′s Modified Eagle′s Medium has been used:

• to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
• to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
• to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells

Preparation Note

Formulated to contain 8.3 grams of powder per liter of medium.

Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations sur la sécurité

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable