For an MTT assay, standard RPMI or DMEM media are suitable options. These include RPMI (Product R0883) and DMEM (Product D5671), both of which may contain up to 10% FBS. When performing assays, such as mitogen or complement-mediated cytotoxicity assays, cells should be suspended in a tissue culture medium, such as DMEM or RPMI, before adding the MTT reagent to each well.
D5030
Dulbecco′s Modified Eagle′s Medium
Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
DME, DMEM
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Niveau de qualité
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Composants
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.
Application
Dulbecco′s Modified Eagle′s Medium has been used:
- to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
- to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
- to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells
Quantité
Formulated to contain 8.3 grams of powder per liter of medium.
Reconstitution
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Supplément
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Protocoles
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
Contenu apparenté
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
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Is there an available variant of EMEM that is free from phenol red, glutamine, and NaHCO3? Additionally, for an MTT assay, would it be necessary to have two types of media – one being the aforementioned EMEM variant and the other for regular culture growth?
1 answer-
Helpful?
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Is the CaCl2 included in Product D5030 in the form of a dihydrate or is it anhydrous?
1 answer-
The formulation utilizes Calcium Chloride Anhydrous rather than the dihydrate form.
Helpful?
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What are the preparation instructions of the 10L product?
1 answer-
As listed on the product detail page in the 'DESCRIPTION' section under 'Reconstitution', this product can be supplemented with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
Helpful?
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How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
0 answers
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