D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Synonyme(s) :
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Forme
solid
Activité spécifique
>=30 units/mg protein (biuret)
Température de stockage
−20°C
Description générale
Research area: Cancer
Application
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.
- impregnated on the strip for formate dehydrogenase assay.
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.
- for evaluation of glucose uptake in cultured muscle cells.
- in lactate dehydrogenase cytotoxicity assay.
Actions biochimiques/physiologiques
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Définition de l'unité
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
Forme physique
Supplied as a lyophilized powder containing trehalose.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Apor Veres-Székely et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
Relationship between insulin sensitivity and gene expression in human skeletal muscle
Parikh HM, et al.
BMC endocrine disorders, 21, 32-32 (2021)
Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
Veres-Szekely A, et al.
International Journal of Molecular Sciences, 23(4), 2119-2119 (2022)
Claudio F Gonzalez et al.
The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique