DMS 454

95062832, human lung, Polygonal

• Synonyme(s) : DMS454 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: DMS 454, 95062832
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Polygonal
• Produits: Calcitonin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian lung, small cell carcinoma

Description de la lignée cellulaire

The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.

Application

Human lung tumour model, hormone secretion

Milieu de culture

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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