DMS 53

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95062823, human lung, Epithelial

• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal no. 60
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Propriétés

• biological source: human lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 95062823-DNA-5UG, pkg of vial of cells 95062823-1VL
• growth mode: Adherent
• karyotype: Modal no. 60
• morphology: Epithelial
• products: Variety of hormones, high level of calcitonin
• receptors: Bombesin (BN), Epidermal growth factor (EGF), Transforming growth factor (TGF-beta), acetylcholine receptor (AChR)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Human lung tumour model

Biochem/physiol Actions

DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17β-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-β (TGF β) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.

Human Caucasian lung small cell carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17

Preparation Note

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.