C2

94101906, rat liver, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: C2, 94101906
• Source biologique: rat liver
• Mode de croissance: Adherent
• Caryotype: Hyperdiploid, 52 Chromosomes
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat hepatoma [HGPRT-]

Description de la lignée cellulaire

Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.

Application

Gene expression analysis.

Milieu de culture

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm² dish.

Autres remarques

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