RO82-W-1

92030502, human thyroid, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: RO82-W-1, 92030502
• Source biologique: human thyroid
• Conditionnement: tube of 5 μg 92030502-DNA-5UG; pkg of vial of cells 92030502-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 60-82, 82 predominant
• Morphologie: Epithelial
• Produits: Thyroglobulin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human follicular thryoid carcinoma

Description de la lignée cellulaire

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

Application

A valuable model for the study of human follicular carcinoma.

Milieu de culture

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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