COLO 205 Cell Line human

87061208, human colon. Round, refractile cells in suspension. Some cuboidal morphology in monolayer.

• Synonyme(s) : COLO205 Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: COLO 205 Cell Line human, 87061208
• Source biologique: human colon
• Description: Human Caucasian colon adenocarcinoma
• Mode de croissance: Suspension
• Caryotype: Hypertriploid modal no 71-78
• Morphologie: Round and refractile cells in suspension. Some cuboidal cells in the monolayer
• Produits: Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Application

tumourigenic in suitable animals

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

Autres remarques

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