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Key Documents

C-23160

Sigma-Aldrich

Skeletal Muscle Cell Growth Medium

Kit including Basal Medium and SupplementPack, 500 ml

Synonyme(s) :

Primary Cell Culture Media

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Forme

liquid

Conditionnement

pkg of 500 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Description générale

Skeletal Muscle Cell Growth Medium Kit

Application

Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Skeletal Muscle Cell Growth Medium are subjected to comprehensive quality control tests using human primary SkMC. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlFetuin (bovine): 50 µg / mlEpidermal Growth Factor (recombinant human): 10 ng / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 10 µg / mlDexamethasone: 0.4 µg / ml

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Primary Human Skeletal Muscle Cells and Growth Media

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

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