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Key Documents

C1059

Cloning cylinders, glass

volume 150 μL

Synonyme(s) :

cloning cylinder, cloning equipment

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About This Item

Code UNSPSC :
41106618
Nomenclature NACRES :
NB.22

Matériaux

light brown (tinted)

Stérilité

sterile; electron beam irradiated

Conditionnement

dish of 15 cylinders

Fabricant/nom de marque

Millipore TR1004

Taille

8 mm × 8 mm

Volume

150 μL

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Catégories apparentées

Description générale

Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.

Conditionnement

Packaged in 60 mm dish with silicone grease on one end

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Tamara D Kolokoltsova et al.
Human cell, 30(4), 249-257 (2017-06-15)
In the present study, we describe a new method of isolation and culture of human villous and extravillous trophoblasts from term placenta. The cultivation of trypsinized placental villous tissue explants, followed by the isolation of cells from outgrowth islets allows
D Geerts et al.
The Journal of cell biology, 147(2), 417-434 (1999-10-20)
Hemidesmosomes are stable adhesion complexes in basal epithelial cells that provide a link between the intermediate filament network and the extracellular matrix. We have investigated the recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin and have shown that the
Maria Chiara Lionetti et al.
Biophysical journal, 118(9), 2319-2332 (2020-04-23)
The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford

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