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Merck

B2025

Brilliant Blue G - Colloidal Concentrate

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A propos de cet article

NACRES:
NA.85
UNSPSC Code:
12352200
Service technique
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General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Preparation Note

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.


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Corrosion

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Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

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Not applicable

flash_point_c

Not applicable

Classe de stockage

12 - Non Combustible Liquids



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